Ginting, Chrismis Novalinda and Lister, I Nyoman Ehrich and Girsang, Ermi and Widowati, Wahyu and Yusepany, Dewani Tediana and Azizah, Alya Mardhotillah and Kusuma, Hanna Sari W. (2021) Hepatotoxicity Prevention in Acetaminophen-induced HepG2 cells by Red Betel (Piper crocatum Ruiz and Pav) Extract from Indonesia via Antioxidant, Anti-Inflammatory, and Anti-Necrotic. Heliyon, 7.
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Abstract
Acetaminophen (APAP) is a widely used analgesic, but it may cause liver injury (hepatotoxicity) via oxidative stress that induced by N-acetyl-p-benzoquinone imine (NAPQI) in long term usage or overdose. Multiple in�flammatory mediators were also found to contribute for this effect. Many medicinal plants was known for its antioxidant and anti-inflammatory activities and one of them is Red betel (Piper crocatum Ruiz and Pav) from Indonesia. In this study, the red betel leaves extract (RBLE) protective effect against APAP-induced HepG2 cells was determined. APAP-induced HepG2 as hepatotoxicity cell model was treated with RBLE at 25 and 100 μg/mL. Protective effects of RBLE toward hepatotoxicity were evaluated by several parameters: tumor necrosis factor-α (TNF-α) concentration, reactive oxygen species (ROS) level, live cells percentage, apoptotic cells percentage, necrotic cells percentage, death cells percentage, CYP2E1 and GPX gene expression. The RBLE treatments (both 25 and 100 μg/mL) increased CYP2E1 and GPX gene expression also live cells percentage, while decreased ROS level, TNF-α concentration, also the percentage of death and necrotic cells. Red Betel leaves ethanol extract has hepatoprotective effect via anti-inflammatory, anti-necrotic, and antioxidant potency in liver injury model.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Red betel leaves extract; Acetaminophen; HepG2 cells; Hepatoprotective; Biochemistry; Immunology; Inflammation; Natural product; Pharmaceutical science |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 15 Sep 2021 04:50 |
| Last Modified: | 14 Nov 2022 04:23 |
| URI: | http://repository.maranatha.edu/id/eprint/27831 |
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