Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of Beluntas Leaf Ethanol Extract against Streptococcus Mutans

Septiani, Elin and Pranata, Natallia and Sugiaman, Vinna Kurniawati (2022) Minimum Inhibitory Concentration and Minimum Bactericidal Concentration of Beluntas Leaf Ethanol Extract against Streptococcus Mutans. Journal of Dentomaxillofacial Science, 7 (2). pp. 83-87. ISSN 2503-0825

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Abstract

Objective: The purpose of this study was to determine the exact values of Minimum Bactericidal Concentration (MBC) and Minimum Inhibitory Concentration (MIC) of beluntas leaf extract against Streptococcus mutans colonies. Material and Methods: This study used Streptococcus mutans ATCC 25175 and ethanol extract of beluntas leaves with concentrations: 250 g/ml, 125 g/ml, 62.5 g/ml, 31.3 g/ml, 15.6 g/ml, 7.8 g /ml, 3.9 g/ml and 1.95μg/ml. This study used two methods, broth microdilution and total plate count techniques as the designated method for the purpose of counting bacteria. Furthermore, the data analysis test was performed with one way ANOVA parametric test to determine the significance between the beluntas leaf extract and the number of Streptococcus mutans bacteria, which will then continued by posthoc test with the Tuckey method to find out which dataset is the most significantly different in among others. hoc test with the Tuckey method to find out which dataset is the most significantly different in among others. Results: The beluntas leaf extract showed significant bacterial properties against Streptococcus mutans (P-Value = 0.00) which was expressed through definite MBC and MIC values. The results showed that the MIC and MBC values were 62.5 g/ml and 125 g/ml, respectively against Streptococcus mutans colonies. Conclusion: The conclusion of this study was that the ethanol extract of the leaves of beluntas (Pluchea Indica L) showed antibacterial properties as indicated by the MIC value at a concentration of 62.5μg/ ml and the MBC at a concentration of 125μg/ml against streptococcus mutans.

Item Type:	Article
Contributors:	Contribution Contributors NIDN/NIDK Email UNSPECIFIED Septiani, Elin UNSPECIFIED UNSPECIFIED UNSPECIFIED Pranata, Natallia UNSPECIFIED UNSPECIFIED UNSPECIFIED Sugiaman, Vinna Kurniawati UNSPECIFIED UNSPECIFIED
Uncontrolled Keywords:	Pluchea indica L., Minimum Bactericidal Concentration, Minimum Inhibitory Concentration, Streptococcus mutans
Subjects:	R Medicine > RK Dentistry
Depositing User:	Perpustakaan Maranatha
Date Deposited:	24 Nov 2023 14:23
Last Modified:	10 Dec 2023 17:22
URI:	http://repository.maranatha.edu/id/eprint/32348

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