Fungistatic effect of Moringa oleifera Lam. on the metabolism changes of Candida albicans

Gani, Basri A. and Soraya, Cut and Sugiaman, Vinna K. and Batubara, Fitri Yunita and Syafriza, Dharli and Naliani, Silvia and Rezeki, Sri and Jakfar, Subhani and Nazar, Muhammad and Hayati, Kemala (2023) Fungistatic effect of Moringa oleifera Lam. on the metabolism changes of Candida albicans. Journal of Pharmacy & Pharmacognosy Research, 11 (1). pp. 179-190. ISSN 0719-4250

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Abstract

Context: Candida albicans is a pathological agent that triggers oral candidiasis because C. albicans can adapt to changes to increase growth and adhesion through biofilm formation mechanisms. Moringa oleifera Lam. has been reported to have fungistatic properties and increases the metabolism change of C. albicans. Aims: To evaluate the fungistatic effect of M. oleifera leaves ethanolic extract (MOLE) on the metabolism changes of C. albicans cells associated with growth and biofilm formation. Methods: The assessment of metabolism changes (stress response and metabolic alterations) of C. albicans by the action of MOLE was performed by mean of FTIR, growth assessment by spectrophotometry, biofilm formation with 1% crystal violet, also read by spectrophotometry, and observation of biofilm mass with a microscope. Results: MOLE showed substantial absorption values based on topological polar surface area (<140 Å2). Concentrations of 25% and 6.25% of MOLE increased the stress response (metabolism changes) of C. albicans (66-75%), meanwhile 50% and nystatin (100.000 IU/mL) were similar in inducing metabolism changes of C. albicans. All concentrations of M. oleifera could inhibit the growth of C. albicans at all incubation times (24, 48, and 72 h) with an Optical Density (OD) of 0.02-0.05 (<300 CFU/mL) and were able to degrade the biofilm formation of C. albicans on a scale substantial at 24 and 48 h (OD>0.4), and moderate scale at 72 h (OD 0.2-0.39). Conclusions: The extract of M. oleifera has increased metabolism changes (stress response) of C. albicans cells, which correlate with the ability to inhibit growth and biofilm formation for 24, 48, and 72 h.

Item Type:	Article
Contributors:	Contribution Contributors NIDN/NIDK Email UNSPECIFIED Gani, Basri A. UNSPECIFIED UNSPECIFIED UNSPECIFIED Soraya, Cut UNSPECIFIED UNSPECIFIED UNSPECIFIED Sugiaman, Vinna K. UNSPECIFIED UNSPECIFIED UNSPECIFIED Batubara, Fitri Yunita UNSPECIFIED UNSPECIFIED UNSPECIFIED Syafriza, Dharli UNSPECIFIED UNSPECIFIED UNSPECIFIED Naliani, Silvia UNSPECIFIED UNSPECIFIED UNSPECIFIED Rezeki, Sri UNSPECIFIED UNSPECIFIED UNSPECIFIED Jakfar, Subhani UNSPECIFIED UNSPECIFIED UNSPECIFIED Nazar, Muhammad UNSPECIFIED UNSPECIFIED UNSPECIFIED Hayati, Kemala UNSPECIFIED UNSPECIFIED
Uncontrolled Keywords:	biofilm; Candida albicans; fungistatic; growth; stress response.
Subjects:	R Medicine > R Medicine (General)
Depositing User:	Perpustakaan Maranatha
Date Deposited:	24 Nov 2023 03:36
Last Modified:	10 Dec 2023 17:32
URI:	http://repository.maranatha.edu/id/eprint/32340

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