The Functional Relationship of Yap/Taz with Autophagy Functions in Sarcopenia Associated with Aging

Sanjaya, Ardo and Lesmana, Ronny and Goenawan, Hanna and Setiawan, Iwan and Sylviana, Nova and Pratiwi, Yuni S. and Dewi, Fitriya N. and Supratman, Unang (2023) The Functional Relationship of Yap/Taz with Autophagy Functions in Sarcopenia Associated with Aging. Nutrition and Healthy Aging, 8 (1). pp. 31-39. ISSN 2451-0502

Text 9. The functional relationship of YapTaz with autophagy functions in sarcopenia associated with aging.pdf Download (2606Kb)

Abstract

BACKGROUND: Muscle loss is one of the features associated with aging with significant impacts on the quality of life. Yap/Taz, the effectors of the Hippo pathway, was shown to regulate organ size and may be associated with aging.We postulate that Yap/Taz modulation may play a role in maintaining muscle fiber size or mediating the function of autophagy during aging and sarcopenia. OBJECTIVE:The research aim to explore sarcopenia and its relationship to autophagy andYap/Taz expression. Additionally, we also explored the relationship of autophagy function and Yap/Taz on skeletal muscle tissue during aging. METHODS: We conducted experiments on two groups of rats kept at 16 and 80 weeks. Skeletal muscle tissue from the soleus muscle was harvested, and mRNA expression of Yap/Taz and genes associated with the autophagy pathway were quantified. Immunoblotting was done with antibodies against Yap/Taz and autophagy proteins. Bafilomycin and Verteporfin were used on the C2C12 cell line to elucidate the interaction between autophagy and Yap/Taz. RESULTS: Old rats were found to have a smaller fiber surface area of the soleus muscle and was associated with increased Yap mRNA and protein expression. The inhibition of autophagy increased Yap levels. However, the inhibition of Yap/Taz function did not affect autophagy in skeletal muscle. CONCLUSIONS: With current evidence, increased Yap was paradoxically associated with sarcopenia, and this increase was caused by the decreased autophagic flux caused by aging.

Item Type:	Article
Contributors:	Contribution Contributors NIDN/NIDK Email UNSPECIFIED Sanjaya, Ardo UNSPECIFIED UNSPECIFIED UNSPECIFIED Lesmana, Ronny UNSPECIFIED UNSPECIFIED UNSPECIFIED Goenawan, Hanna UNSPECIFIED UNSPECIFIED UNSPECIFIED Setiawan, Iwan UNSPECIFIED UNSPECIFIED UNSPECIFIED Sylviana, Nova UNSPECIFIED UNSPECIFIED UNSPECIFIED Pratiwi, Yuni S. UNSPECIFIED UNSPECIFIED UNSPECIFIED Dewi, Fitriya N. UNSPECIFIED UNSPECIFIED UNSPECIFIED Supratman, Unang UNSPECIFIED UNSPECIFIED
Uncontrolled Keywords:	Aging, autophagy, Hippo pathway, sarcopenia, yes-associated protein, skeletal muscle
Subjects:	R Medicine > R Medicine (General)
Depositing User:	Perpustakaan Maranatha
Date Deposited:	13 Jul 2023 09:18
Last Modified:	13 Jul 2023 09:18
URI:	http://repository.maranatha.edu/id/eprint/32002

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