Widowati, Wahyu and Wargasetia, Teresa Liliana and Rahardja, Fanny and Gunanegara, Rimonta F. and Priyandoko, Didik and Gondokesumo, Marisca Evalina and Afifah, Ervi and Wijayanti, Cahyaning Riski and Rizal, Rizal (2022) Human Wharton’s Jelly Mesenchymal Stem Cells Inhibit Cytokine Storm in Acute Respiratory Distress Syndrome in a Rat Model. Asian Pacific Journal of Tropical Biomedicine, 12 (8). pp. 323-366. ISSN 2588-9222
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Abstract
Objective: To evaluate the potential effect of human Wharton’s jelly mesenchymal stem cells (hWJMSCs) on acute respiratory distress syndrome in lipopolysaccharide (LPS)-induced rats. Methods: The hWJMSCs (5×104 /mL, 5×105 /mL, 5×106 /mL) were administered to rats on day 1 and day 8 after being induced by LPS (5 mg/kg body weight). TNF-α levels in the lung and IL�18 and IL-1β levels in the serum were measured using ELISA. In addition, caspase-1 expression in lung tissues was quantified using qRT-PCR, and NF-κB and IL-6 expressions were assessed using immunohistochemistry. Results: The hWJMSCs decreased TNF-α levels in the lung and plasma IL-18 and IL-1β levels. Moreover, the hWJMSCs downregulated the expressions of caspase-1, IL-6, and NF-κB in lung tissues. Conclusions: The hWJMSCs can decrease inflammatory markers of acute respiratory distress syndrome in a rat model and may be further investigated for the treatment of acute respiratory distress syndrome.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Human Wharton’s jelly mesenchymal stem cells; Acute respiratory distress syndrome; TNF-α; IL-18; NF-κB; Inflammatory marker |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 04 Feb 2023 01:17 |
| Last Modified: | 04 Feb 2023 01:17 |
| URI: | http://repository.maranatha.edu/id/eprint/31281 |
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