Antibacterial Activity Of Clove Bud Essential Oil (Syzygium Aromaticum) As Coolant Agent In Dental Ultrasonic Scaler Against Actinobacillus Actinomycetemcomitans

Saputri, Rosalina I. and Nuryanti, Archadian and Utami, Trianna W. (2021) Antibacterial Activity Of Clove Bud Essential Oil (Syzygium Aromaticum) As Coolant Agent In Dental Ultrasonic Scaler Against Actinobacillus Actinomycetemcomitans. Biochemical and Cellular Archives, 21 (2). pp. 3301-3305. ISSN 0976-1772

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Abstract

Dental clinicians prefer the use of ultrasonic scalers over manual scalers for dental treatment. The aim of this study is to examine the concentration of clove bud essential oil (CBEO) as an ultrasonic scaler coolant that could inhibit the growth of Actinobacillus actinomycetemcomitans, a key periodontal pathogen. Three replicates of 5 mL of 0.04%, 0.08%, 0.16%, 0.32%, and 0.64% CBEO, 5% PEG and 0.0005% chlorhexidine gluconate mixed with an ultrasonic scaler were sprayed into A. actinomycetemcomitans cultures (1.5 × 104 colony forming units/mL in 5 mL of 0.9%NaCl). A dilution of the bacterial–antibacterial mixture (200 µL) was inoculated on blood agar plates and then incubated for 3 × 24 h. Inhibitory effects were examined by the colony count method. The Kruskal–Wallis test showed significant differences in the antibacterial activity of different CBEO concentrations (p < 0.05). Multiple comparisons showed significant differences (1) between the negative control and 0.04% and 0.08% CBEO and (2) between 0.64% CBEO and the positive control. No significant difference was observed between 0.16%, 0.32% and 0.64% CBEO and the positive control. In conclusion, CBEO has potential application as an antibacterial ultrasonic scaler coolant. Further research on the optimum concentration and toxicity of CBEO is necessary.

Item Type: Article
Uncontrolled Keywords: Actinobacillus actinomycetemcomitans, antibacterial, bacterial inhibition, clove bud essential oil, dental scaling, ultrasonic scaler coolant
Subjects: R Medicine > RK Dentistry
Depositing User: Perpustakaan Maranatha
Date Deposited: 08 Sep 2022 05:40
Last Modified: 08 Sep 2022 05:40
URI: http://repository.maranatha.edu/id/eprint/30456

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