Ratnawati, Hana and Wargasetia, Teresa Liliana and Sandra, Ferry (2018) The Expression of miRNA-21 and miRNA-18a in Nasopharyngeal Cancer: a Possibility as Non-invasive Biomarker. In: International Symposium on Cancer Biology, 24-25 Agustus 2018, Yogyakarta.
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6. The expression of miRNA-21 and miRNA-18a in NPC.pdf Download (805Kb) | Preview |
Abstract
Nasopharyngeal cancer, the most common head and neck cancer in Indonesia, is difficult to detect in the early stages due to unclear symptoms and hidden locations. This causes more than 70% of patients come at an advanced stage and with metastasis. Studies show that miRNA regulates more than 60% of protein coding genes, thus regulating almost all biological processes within the cell including the pathogenesis of cancer. It turns out that miRNA not only plays a role in the cell, but also can be found in blood circulation and other body fluids, thus it is a potential biomarker for early detection of various types of cancer. MiR-21 is one of the oncogene miRNA that regulates genes that play a role in cell proliferation, apoptosis and angiogenesis. MiRNA-18a is a miRNA associated with Epstein-Barr virus, which plays an important role in the pathogenesis of nasopharyngeal cancer. The purpose of this study was to determine the expression of miRNA-21 and mi-RNA-18a in nasopharyngeal cancer cell lines and analysis the possibility to become a non-invasive potential biomarker that could predict the presence of nasopharyngeal cancer at an early stage. This is an experimental laboratory study using EBV-positive nasopharyngeal cancer cell line, C666 and normal nasopharyngeal cell line, NP69. Total RNA was isolated from cell culture and RNA was transcribed back to cDNA then continued with real-time qPCR. The cycle threshold value is calculated to quantify the relative amounts of miR-21 and miR-18a. The result showed that there is a significant difference of miRNA-18a (p=0,000) and miRNA-21 (p=0,002) expression on nasopharyngeal cell (C666) and normal nasopharynx cell (NP69). It can be concluded that, miRNA-18a and miRNA-21 could play a role as non-invasive biomarker in nasopharyngeal cancer.
| Item Type: | Conference or Workshop Item (Paper) |
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| Uncontrolled Keywords: | nasopharyngeal cancer, miR-21, miR-18a, biomarker |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 14 Aug 2022 13:02 |
| Last Modified: | 14 Aug 2022 13:02 |
| URI: | http://repository.maranatha.edu/id/eprint/30434 |
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