Widowati, Wahyu and Gunanegara, Rimonta F. and Wargasetia, Teresa Liliana and Kusuma, Hanna Sari Widya and Arumwardana, Seila and Wahyuni, Cintani Dewi and Girsang, Ermi and Lister, I Nyoman Ehrich and Ginting, Chrismis Novalinda and Rizal, Rizal and Bachtiar, Indra and Murti, Harry and Kim, Young Ho (2021) Effect of Flavonoids on Oxidative Stress, Apoptosis, and Cell Makers of Peripheral Blood-Derived Endothelial Progenitor Cells: An In Vitro Study. International Journal of Applied Pharmaceutics, 13 (3). pp. 39-42. ISSN 0975-7058
|
Text
Int Publish 2021-3 (Wahyu IJAP-Wahyu FKUKM).pdf Download (253Kb) | Preview |
Abstract
Objective: Circulating EPCs (endothelial progenitor cells) play a role in neovascularization and vascular repair. Oxidative stress impairs endothelial progenitor. Flavonoid is a phytochemical compound for antioxidant activity. Flavonoid effects toward oxidative stress, apoptosis, and expression of the cell markers on EPCs are not fully understood. This study was aimed to elucidate the effects of quercetin, kaempferol, and myricetin toward oxidative stress, apoptosis, and cell markers of peripheral blood-derived-EPCs. Methods: EPCs (endothelial progenitor cells) were isolated from peripheral blood mononuclear cells (PBMNCs) using cultivation under EPCs spesific media. Oxidative stress in EPCs was induced by H2O2 and then treated by quercetin, kaempferol, and myricetin. Cytotoxicity was measured by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, while intracellular reactive oxygen species (ROS), apoptosis and characterization of cells, which expressed CD133 and KDR, was measured using flow cytometry. Results: Quercetin, kaempferol, and myricetin at concentration 12.50 μmol/l were not toxic on EPCs as the cells viability were 96.11±4.03%, 95.42±7.75%, and 94.22±9.49%, respectively. Flavonoids decreased intracellular ROS level in EPCs (quercetin: 14.38±1.47%, kaempferol: 20.21±6.25%, and myricetin: 13.88±4.02%) compared to EPCs treated with H2O2 (30.70%±1.04). Percetage of EPCs apoptosis was not significantly different among each treatment. Immunophenotyping showed the increasing of CD133 and KDR expression in EPCs treated with flavonoids. Conclusion: Quercetin, kaempferol, and myricetin were safe for EPCs, decreased ROS levels, and increased CD133 and KDR expression. However, the flavonoids did not significantly affect EPCs apoptosis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Apoptosis, Endothelial progenitor cells, Flavonoids, Reactive oxygen species |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 24 Mar 2022 05:00 |
| Last Modified: | 24 Mar 2022 05:00 |
| URI: | http://repository.maranatha.edu/id/eprint/30176 |
Actions (login required)
 |
View Item |
