Marlina, Marlina and Armenia, Armenia and Aviani, Jennifer Kiem and Sholihah, Ika Adhani and Kusuma, Hanna Sari W. and Azizah, Alya Mardhotillah and Elida, Nur and Widowati, Wahyu (2021) Conditioned medium of IGF1-induced synovial membrane mesenchymal stem cells increases chondrogenic and chondro protective markers in chondrocyte inflammation. Bioscience Reports, 41.
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Abstract
Recently,mesenchymalstemcells(MSCs)have been the most explored cells for cell therapy for osteoarthritis(OA) that can be obtained from various sources.Synovial membrane MSCs (SMMSCs) provide bestpotentialforOAtherapy,howevertheyarenotwidelyexplored.Con- ditioned mediumofSMMSCs(SMMSCs-CM)richingrowthfactorsandcytokinescaninhibit apoptosis andincreasechondrocytescellproliferation.Theaimofthepresentstudywasto determine growthfactorscontentinSMMSCs-CMaswellasthechondrogenicandchon- droprotectivemarkersexpressioninOAmodelafterinsulin-likegrowthfactor(IGF)1-induced and non-inducedSMMSCs-CMtreatments.Chondrocytecellline(CHON002)wasinduced by IL1β as OAmodel(CHON002withIL1β (IL1β-CHON002)) andtreatedwithSMMSCs-CM with orwithoutIGF1inductiontodetermineitseffectivenessinrepairingOAcellsmodel. ELISA wasusedtoassayBMP2,fibroblastgrowthfactor18(FGF18)andtransforming growthfactor(TGF) β1 (TGFβ1) levelsinSMMSCs-CM,matrixmetalloproteinase(MMP)13 (MMP13) andadisintegrinandmetalloproteinasewiththrombospondinmotif4(ADAMTS4) levels inOAcellsmodeltreatedwithSMMSCs-CM.RT-qPCRanalyseswereusedtoinves- tigate thegeneexpressionofSOX9,COL2,andCOL10.CMfromSMMSCsculturedand induced byIGF1150ng/mLwasthemosteffectiveconcentrationforincreasingthecontent of growthfactormarkersofSMMSCs-CM,whichhadsuccessfullyincreasednegativecar- tilage hypertrophymarkers(SOX9andCOL2)andreducedhypertrophymarkers(COL10, MMP13, andADAMTS4).PreconditioningwithIGF1hasbetterandverysignificantresults in loweringMMP13andADAMTS4levels.ThepresentstudysupportsIGF1pre-conditioned SMMSCs-CM todevelopanewtherapeuticapproachinOAimprovementthroughitschon- drogenicandchondroprotectiveroles.
| Item Type: | Article |
|---|---|
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 15 Sep 2021 03:43 |
| Last Modified: | 14 Nov 2022 05:16 |
| URI: | http://repository.maranatha.edu/id/eprint/27823 |
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