Antonius, Andreas Ardhika and Widowati, Wahyu and Wijaya, Laura and Agustina, Dwi and Puradisastra, Sugiarto and Sumitro, Sutiman B. and Widodo, M. Aris and Bachtiar, Indra (2015) Human Platelet Lysate Enhances the Proliferation of Wharton’s Jelly-Derived Mesenchymal Stem Cells. Biomarkers and Genomic Medicine, 7 (3). pp. 87-97. ISSN 2214-0247
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Abstract
This study was performed to elucidate the potential of human platelet lysate (huPL) as an alternative to xeno-free media for culturing mesenchymal stem cells (MSCs). In this work, Wharton’s jelly-derived MSCs (WJ-MSCs; n Z 5) were isolated, characterized, and cultured in huPL derived from type O platelets reconstituted with type AB platelet-poor plasma (huPLeABO). WJ-MSCs from five donors were cultured under two different conditions [cell culture supplemented with 20% fetal bovine serum (FBS) and 0.250 mg/mL, 0.125 mg/ mL, 0.063 mg/mL, and 0.031 mg/mL huPL]. Growth kinetics, cell surface markers, and in vitro differentiation potential toward the adipogenic, chondrogenic, and osteogenic lineages were evaluated. Our results indicate that WJ-MSCs cultured in the presence of huPLeABO showed typical fibroblast-like morphology and had a significantly higher cell count (p < 0.05), compared with those cultured in FBS. Furthermore, results of immunophenotyping assay showed similar MSCs characteristics for both culture conditions. In addition, no significant differences were observed in the MSCs differentiation toward the adipogenic, chondrogenic, and osteogenic lineages. The optimal concentrations of huPLeABO in the culture medium were 0.250 mg/mL and 0.125 mg/mL. Because of insignificant differences between the concentrations in terms of WJ-MSCs population doubling time, the concentration of 0.125 mg/mL was used in all comparisons between FBS and huPLeABO. The average concentrations of insulinlike growth factor-1, platelet-derived growth factor-AB, vascular endothelial growth factor, and transforming growth factor-b1 in huPLeABO were 287.89 pg/mg, 47,096.63 pg/mg,150.93 pg/mg, and 74,817.76 pg/mg, respectively. huPLeABO produced by this method was able to enhance the WJ-MSCs proliferation rate constantly and decrease the required time to reach confluence compared with the FBS culture condition.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | fetal bovine serum; human platelet lysate; population doubling time; Wharton’s jelly derivedmesenchymal stem cells; xeno-free media |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 06 Oct 2020 05:07 |
| Last Modified: | 14 Nov 2022 03:19 |
| URI: | http://repository.maranatha.edu/id/eprint/27377 |
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