Widowati, Wahyu and Widyanto, Rahma Micho and Husin, Winsa and Ratnawati, Hana and Laksmitawati, Dian R. and Setiawan, Bambang and Nugrahenny, Dian and Bachtiar, Indra (2014) Green Tea Extract Protects Endothelial Progenitor Cells from Oxidative Insult through Reduction of Intracellular Reactive Oxygen Species Activity. Iranian Journal of Basic Medical Sciences, 17 (9). pp. 702-709. ISSN 2008-3874
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Abstract
Objective(s): Many studies have reported that tea consumption decreases cardiovascular risk, but the mechanisms remain unclear. Green tea is known to have potent antioxidant and free radical scavenging activities. This study aimed to investigate whether green tea extract (GTE) can protect endothelial progenitors cells (EPCs) against oxidative stress through antioxidant mechanisms. Materials and Methods: Mononuclear cells (MNCs) were isolated from peripheral blood by density gradient centrifugation with Ficoll. The cells were then plated on fibronectin-coated culture dishes. After 7 days of culture, EPCs were characterized as adherent cells double positive for DiI-ac-LDL uptake and lectin binding. EPCs were further identified by assessing the expression of CD34/45, CD133, and KDR. EPCs were then treated with hydrogen peroxide (H2O2) at doses of 50, 100, 200 μM and incubated with or without GTE (25 μg/ml). The intracellular reactive oxygen species (ROS) levels were detected by flow cytometry using a 2’,7’-dichlorofluorescein diacetate (DCF-DA) fluorescent probe. Results: GTE ameliorated the cell viability of EPCs induced by H2O2 at doses of 50, 100, 200 μM for about 25.47, 22.52, and 11.96% higher than controls, respectively. GTE also decreased the intracellular ROS levels of EPCs induced by H2O2 at doses of 50, 100, 200 μM for about 84.24, 92.27, and 93.72% compared to controls, respectively. Conclusion: GTE improves cell viability by reducing the intracellular ROS accumulation in H2O2-induced EPCs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Antioxidant Endothelial progenitor cells Green tea Oxidative stress Reactive oxygen species |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 10 Aug 2015 08:53 |
| Last Modified: | 14 Nov 2022 01:38 |
| URI: | http://repository.maranatha.edu/id/eprint/14418 |
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