Protein Hydrolysate of Green Peas Bromelain Attenuates Kidney Fibrosis in Cisplatin-Induced Nephrotoxicity Rats: Emphasis on Anti-Inflammatory Activities

Hidayat, Meilinah and Pangastuti, Retno and Prahastuti, Sijani and Hasan, Khomaini (2023) Protein Hydrolysate of Green Peas Bromelain Attenuates Kidney Fibrosis in Cisplatin-Induced Nephrotoxicity Rats: Emphasis on Anti-Inflammatory Activities. Hayati: Journal of Biosciences, 30 (2). pp. 347-357. ISSN 1978-3019

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Abstract

Massive tubule fibrosis is a histopathology hallmark of chronic renal failure (CRF). The previous study of protein hydrolysate of green pea (Pisum sativum) bromelain (PHGPB) showed promising results as an antifibrosis in glucoseinduced renal mesangial culture cells, by decreasing their TGF-β levels. In this experiment the anti-inflammatory, anti-fibrotic effect of PHGPB in Cisplatininduced chronic renal failure (CRF) rats was measured. The purpose of this study is to evaluate the anti-inflammatory and antifibrosis effect of PHGPB in Cisplatin-induced chronic renal failure rats. Five groups consisted of five rats: negative control, Cisplatin control, and three groups of Cisplatin+PHGPB (dose of 100, 200, and 400 mg/kg BW/day) treated for 56 days. The examined parameters are Beta-2-microglobulin (β2MG), hs-CRP, histopathological observations of Masson's trichrome stain, and IHC. The level of β2MG and hs-CRP in the Cisplatin+PHGPB group lowered and was highly different from Cisplatin control (p<0.05) depending on doses. In histopathology, the Cisplatin+PHGPB 400 mg/ kg BW group showed less fibrosis and no significant difference with the negative control in Masson trichome staining. While in IHC histopathology, PHGPB treatment slightly ameliorated TGF-β expression and intensity of TGF-β values convincingly. In conclusion, PHGPB can relieve kidney fibrosis in cisplatininduced nephrotoxicity rats: Emphasis on anti-inflammatory activities.

Item Type:	Article
Contributors:	Contribution Contributors NIDN/NIDK Email UNSPECIFIED Hidayat, Meilinah UNSPECIFIED UNSPECIFIED UNSPECIFIED Pangastuti, Retno UNSPECIFIED UNSPECIFIED UNSPECIFIED Prahastuti, Sijani UNSPECIFIED UNSPECIFIED UNSPECIFIED Hasan, Khomaini UNSPECIFIED UNSPECIFIED
Uncontrolled Keywords:	Beta-2-microglobulin, green pea, hs-CRP, immunohistochemistry, TGF-β
Subjects:	R Medicine > R Medicine (General)
Depositing User:	Perpustakaan Maranatha
Date Deposited:	26 Jul 2023 03:38
Last Modified:	26 Jul 2023 03:38
URI:	http://repository.maranatha.edu/id/eprint/32012

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