Sanjaya, Ardo and Lesmana, Ronny and Goenawan, Hanna and Setiawan, Iwan and Sylviana, Nova and Pratiwi, Yuni S. and Dewi, Fitriya N. and Supratman, Unang
(2023)
The Functional Relationship of Yap/Taz with Autophagy Functions in Sarcopenia Associated with Aging.
Nutrition and Healthy Aging, 8 (1).
pp. 31-39.
ISSN 2451-0502
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9. The functional relationship of YapTaz with autophagy functions in sarcopenia associated with aging.pdf
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Abstract
BACKGROUND: Muscle loss is one of the features associated with aging with significant impacts on the quality of life.
Yap/Taz, the effectors of the Hippo pathway, was shown to regulate organ size and may be associated with aging.We postulate
that Yap/Taz modulation may play a role in maintaining muscle fiber size or mediating the function of autophagy during
aging and sarcopenia.
OBJECTIVE:The research aim to explore sarcopenia and its relationship to autophagy andYap/Taz expression. Additionally,
we also explored the relationship of autophagy function and Yap/Taz on skeletal muscle tissue during aging.
METHODS: We conducted experiments on two groups of rats kept at 16 and 80 weeks. Skeletal muscle tissue from the
soleus muscle was harvested, and mRNA expression of Yap/Taz and genes associated with the autophagy pathway were
quantified. Immunoblotting was done with antibodies against Yap/Taz and autophagy proteins. Bafilomycin and Verteporfin
were used on the C2C12 cell line to elucidate the interaction between autophagy and Yap/Taz.
RESULTS: Old rats were found to have a smaller fiber surface area of the soleus muscle and was associated with increased
Yap mRNA and protein expression. The inhibition of autophagy increased Yap levels. However, the inhibition of Yap/Taz
function did not affect autophagy in skeletal muscle.
CONCLUSIONS: With current evidence, increased Yap was paradoxically associated with sarcopenia, and this increase
was caused by the decreased autophagic flux caused by aging.
| Item Type: |
Article
|
| Contributors: |
| Contribution | Contributors | NIDN/NIDK | Email |
|---|
| UNSPECIFIED | Sanjaya, Ardo | UNSPECIFIED | UNSPECIFIED | | UNSPECIFIED | Lesmana, Ronny | UNSPECIFIED | UNSPECIFIED | | UNSPECIFIED | Goenawan, Hanna | UNSPECIFIED | UNSPECIFIED | | UNSPECIFIED | Setiawan, Iwan | UNSPECIFIED | UNSPECIFIED | | UNSPECIFIED | Sylviana, Nova | UNSPECIFIED | UNSPECIFIED | | UNSPECIFIED | Pratiwi, Yuni S. | UNSPECIFIED | UNSPECIFIED | | UNSPECIFIED | Dewi, Fitriya N. | UNSPECIFIED | UNSPECIFIED | | UNSPECIFIED | Supratman, Unang | UNSPECIFIED | UNSPECIFIED |
|
| Uncontrolled Keywords: |
Aging, autophagy, Hippo pathway, sarcopenia, yes-associated protein, skeletal muscle |
| Subjects: |
R Medicine > R Medicine (General) |
| Depositing User: |
Perpustakaan Maranatha
|
| Date Deposited: |
13 Jul 2023 09:18 |
| Last Modified: |
13 Jul 2023 09:18 |
| URI: |
http://repository.maranatha.edu/id/eprint/32002 |
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