Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line

Widowati, Wahyu and Mozef, Tjandrawati and Risdian, Chandra and Ratnawati, Hana and Tjahjani, Susy and Sandra, Ferry (2010) Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line. Indonesian Journal of Cancer Chemoprevention, 1 (2). pp. 99-107. ISSN 2088-0197

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Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of C. roseus in inducing apoptosis on breast cancer cell lin (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-GI flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 mg/ml. Induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 mg/ml induced apoptosis for 22.235%. Meanwhile doxorubicin at concentration of 10 mg/ml induced apoptosis for 36.055%. The antioxidant activity was determined by using in vitro assay: inhibition of 2.2-diphenyl-l-picyrlhydrazyl (DPPH) scavenging activity. Antioxidant activity of C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls. The results showed that DPPH ICso of C. roseus extract, quercetin and BHA were 358.411 mg/ml, 19.200 mg/ml and 94.178 mg/ml, respectively. We suggest that C. roseus extract had a potential anticancer activity by inducing apoptosis.

Item Type: Article
Uncontrolled Keywords: antioxidant, DPPH, Catharanthus roseus, apoptosis, breast cancer, T47D
Subjects: R Medicine > R Medicine (General)
Depositing User: Perpustakaan Maranatha
Date Deposited: 01 Aug 2013 06:27
Last Modified: 01 Aug 2013 06:27
URI: http://repository.maranatha.edu/id/eprint/3828

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