Widowati, Wahyu and Sardjono, Caroline Tan and Wijaya, Laura and Laksmitawati, Dian R. and Pawitan, Jeanne Adiwinata and Sandra, Ferry (2014) Effect of B-carotene on Cell Proliferation and Differentiation of Adipose-derived Stem Cells into Endothelial Progenitor Cells. Biotechnology An Indian Journal, 9 (10). pp. 407-412. ISSN 0974-7435
|
Text
1.Int Publikasi 2014-2 (Wahyu BTAIJ-Wahyu FKUKM) Reviewer.pdf Download (3419Kb) | Preview |
|
|
Text
Turnitin_Int Publikasi 2014-5 (Wahyu BTAIJ-Wahyu FKUKM).pdf.pdf Download (1839Kb) | Preview |
Abstract
Aim: To evaluate the ability of â-carotene to protect oxidation effect of H2O2, and to enhance proliferation and differentiation into AD-MSCs into EPCs. Experimental: AD-MSCs were induced by EGM Bullet Kit medium with or without â-carotene. The proliferation of EPCs was determined by viable cell number counts. Differentiation into EPCs was characterized by the following surface markers: CD 34, CD133 and vascular endothelial growth factor receptor 2 (VEGFR-2). The protective effect of â-carotene was measured base on the level of intracellular reactive oxygen species (ROS) by fluorescence with 2�,7�- dichlorofluorescein diacetate (DCF-DA) using flow cytometry. Results: â-Carotene enhanced cell proliferation and differentiation of AD-MSCs into EPCs, and also decreased the accumulation of H202-induced intracellular ROS in EPCs. Conclusion: â- Carotene plays a role in cell proliferation and differentiation of AD-MSCs to EPCs that might be due to decreased intracellular ROS level.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Endothelial progenitor cell; Oxidative stress; Reactive oxygen species (ROS); â-carotene; Antioxidant; Endothelium. |
| Subjects: | R Medicine > RS Pharmacy and materia medica |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 28 Mar 2014 03:12 |
| Last Modified: | 05 Nov 2021 04:30 |
| URI: | http://repository.maranatha.edu/id/eprint/5288 |
Actions (login required)
 |
View Item |
