Effect of B-carotene on Cell Proliferation and Differentiation of Adipose-derived Stem Cells into Endothelial Progenitor Cells

Widowati, Wahyu and Sardjono, Caroline Tan and Wijaya, Laura and Laksmitawati, Dian R. and Pawitan, Jeanne Adiwinata and Sandra, Ferry (2014) Effect of B-carotene on Cell Proliferation and Differentiation of Adipose-derived Stem Cells into Endothelial Progenitor Cells. Biotechnology An Indian Journal, 9 (10). pp. 407-412. ISSN 0974-7435

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PR 1. Effect of ,B-carotene on cell proliferation and differentiation of adipose-derived stem cells.pdf

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Abstract

Aim: To evaluate the ability of â-carotene to protect oxidation effect of H2O2, and to enhance proliferation and differentiation into AD-MSCs into EPCs. Experimental: AD-MSCs were induced by EGM Bullet Kit medium with or without â-carotene. The proliferation of EPCs was determined by viable cell number counts. Differentiation into EPCs was characterized by the following surface markers: CD 34, CD133 and vascular endothelial growth factor receptor 2 (VEGFR-2). The protective effect of â-carotene was measured base on the level of intracellular reactive oxygen species (ROS) by fluorescence with 2�,7�- dichlorofluorescein diacetate (DCF-DA) using flow cytometry. Results: â-Carotene enhanced cell proliferation and differentiation of AD-MSCs into EPCs, and also decreased the accumulation of H202-induced intracellular ROS in EPCs. Conclusion: â- Carotene plays a role in cell proliferation and differentiation of AD-MSCs to EPCs that might be due to decreased intracellular ROS level.

Item Type: Article
Uncontrolled Keywords: Endothelial progenitor cell; Oxidative stress; Reactive oxygen species (ROS); â-carotene; Antioxidant; Endothelium.
Subjects: R Medicine > RS Pharmacy and materia medica
Depositing User: Perpustakaan Maranatha
Date Deposited: 28 Mar 2014 03:12
Last Modified: 13 Jul 2021 07:23
URI: http://repository.maranatha.edu/id/eprint/5288

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