Widowati, Wahyu and Herlina, Tati and Ratnawati, Hana and Mozef, Tjandrawati and Immanuel, Victor (2011) Potency of Antioxidant, Anticholesterol and Platelet Antiaggregation of Black Tea (Camelia sinensis ). Buletin Penelitian Tanaman Rempah dan Obat, 22 (1). pp. 74-83.
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Abstract
Epidemiological studies have shown an inverse correlation between diets rich in polyphenols, flavonoids and reduced risk of cardiovascular disease. These associations were mainly ascribed to the antioxidant, antiaggregation and anticholesterol capacity of polyphenols. This association has been explained that atherogenesis is initiated by hypercholesterol, lipid peroxidation and hyper aggregation platelet. The research was carried out to evaluate antioxidant, anticholesterol and antiaggregation activities of methanol extract and fractions of black tea (Camelia sinensis). To evaluate antioxidant activity of methanol extract and fractions were compared with (-)-Epigallocatechine 3-gallate (EGCG), anticholesterol activity were compared with simvastatin and antiaggregation activity were compared with aspirin. Antioxidant activity using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenging activity at two concentrations 100 μg/mL, 50 μg/mL and anticholesterol was cholesterol oxidase activity at two concentrations 0.005 μg/mL, 0.0025 μg/mL, the antiaggregation activity used epinephrine (EPN) agonist at two concentrations 300 μM, 75 μM and one concentration 37.1 μg/mL of methanol extract and fractions of black tea. To know the difference of treatment, the data were analysed using Analysis of Variance (ANOVA) and be continued Duncan’s new multiple range test. The results showed that DPPH scavenging activity were high (88.59-93.556%), the anticholesterol showed high activity (93.663- 97.434%) and the antiaggregation showed low up to high activity (79.967- 4.31%). Using Duncan’s new multiple range test showed that anti-oxidant activity were not different among extract and fractions of black tea, the highest anticholesterol activity was black tea extract 0.005 μg/mL (97.434%) and the highest antiaggregation activity was ethyl acetate fraction on 75 μM EPN agonist (4.310 %), water fraction 37.1 μg/mL with 300 μM EPN agonist (21.833%).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Black tea, Camelia sinensis, antioxidant, anticholesterol, antiaggregation, flavonoids, cardiovascular disease |
| Subjects: | R Medicine > RM Therapeutics. Pharmacology |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 15 Jul 2013 04:09 |
| Last Modified: | 15 Jul 2013 04:09 |
| URI: | http://repository.maranatha.edu/id/eprint/3729 |
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