Antioxidant, anticancer, and apoptosis-inducing effects of Piper extracts in HeLa cells

Widowati, Wahyu and Wijaya, Laura and Wargasetia, Teresa Liliana and Bachtiar, Indra and Yellianty, Yellianty and Laksmitawati, Dian R. (2013) Antioxidant, anticancer, and apoptosis-inducing effects of Piper extracts in HeLa cells. Journal of Experimental and Integrative Medicine, 3 (3). pp. 225-230. ISSN 1309-4572

Antioxidant, Anticancer, and Apoptosis-inducing Effect of Piper Extracts in Hela Cells.pdf - Published Version

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Objective: Cervical cancer is the second most common cancer as well as one of leading cause of cancer-related death for women worldwide. In regards to that issue, focus of this paper will be on popularly used Piperaceae members including Piper betle L, Piper cf fragile Benth, Piper umbellatum L, Piper aduncum L, Piper pellucidum L. This research was conducted to elucidate the antioxidant, anticancer and apoptosis inducing activities of Piperaceae extracts on cervical cancer cells, namely HeLa cell line. Methods: The anticancer activity was determined by inhibiting the proliferation of cells. Apoptosis inducing was determined by inhibiting proliferation cells and by SubG1 flow cytometry. The antioxidant activity is determined by using superoxide dismutase value and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Results: The highest anticancer activity at 24 h incubation was found for P.pellucidum extract (IC50: 2.85 μg/ml); The anticancer activity at 48 h incubation was more than at 24 h for all extracts. The highest apoptotic activity was found for P.betle (12.5 μg/ml) at both 24 and 48 h incubatio. The highest antioxidant activity was also represented by P.betle extract. Conclusions: All Piperaceae extracts have high anticancer activity; longer incubation increase anticancer activity. P.betle extract has the highest antioxidant property.

Item Type: Article
Uncontrolled Keywords: Anticancer; Antioxidant; Apoptosis; Cervical cancer; HeLa cell line; Piperaceae
Subjects: R Medicine > RM Therapeutics. Pharmacology
Depositing User: Perpustakaan Maranatha
Date Deposited: 15 Jul 2013 03:21
Last Modified: 04 Feb 2023 00:08

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