Isolation and Characterization of Fibroblast from Normal and Thalassemia Foreskin

Widowati, Wahyu and Faried, Ahmad and Susanah, Susi and Priyandoko, Didik and Sugiaman, Vinna K. and Nainggolan, Ita Margaretha (2024) Isolation and Characterization of Fibroblast from Normal and Thalassemia Foreskin. Trends in Sciences, 21 (6).

	Text 40. TIS. 2024_ 21(6)_ 1-9.pdf Download (12Mb)
	Text 40. Turnitin - Isolation and Characterization of Fibroblast from Normal and Thalassemia Foreskin (1).pdf Download (2646Kb)

Abstract

Thalassemia is a genetic blood disorder impacting hemoglobin production, varies in severity, and requires lifelong treatments. The advancement in somatic cell reprogramming through induced pluripotent stem cells (iPSCs) represents a personalized medicine approach that holds promise as a treatment for individuals with thalassemia. One source that could be reprogrammed into iPSCs can be generated from foreskin fibroblast cells. This study aimed to isolate and characterize human fibroblast cells from normal (NFF) and thalassemia (TFF) foreskin surface markers (CD44, CD90, CD105, CD73), and negative lineage (CD45, CD34, CD11b, CD19, HLA-DR). Fibroblast cells were isolated out of normal and thalassemia foreskin using the explant method. Characterization of NFF and TFF isolates was carried out using flow cytometry to disclose the expression of CD90, CD44, CD73, CD105, and negative lineage. Isolation of fibroblast cells from normal and thalassemia foreskin was successfully carried out using the explant method with NFF and TFF characterized as expressing CD90, CD44, CD105, CD73, and negative lineages (CD45, CD11b, CD34, CD19, and HLA-DR). This result could lead to a continuation of reprogramming into iPSCs.

Item Type:	Article
Contributors:	Contribution Contributors NIDN/NIDK Email Author Widowati, Wahyu UNSPECIFIED UNSPECIFIED Author Faried, Ahmad UNSPECIFIED UNSPECIFIED Author Susanah, Susi UNSPECIFIED UNSPECIFIED Author Priyandoko, Didik UNSPECIFIED UNSPECIFIED Author Sugiaman, Vinna Kurniawati UNSPECIFIED UNSPECIFIED Author Nainggolan, Ita Margaretha UNSPECIFIED UNSPECIFIED
Uncontrolled Keywords:	Characterization, Fibroblast, Flow cytometry, Isolation, Thalassemia
Subjects:	R Medicine > R Medicine (General)
Depositing User:	Perpustakaan Maranatha
Date Deposited:	08 Sep 2024 10:15
Last Modified:	08 Sep 2024 10:18
URI:	http://repository.maranatha.edu/id/eprint/33386

Actions (login required)

View Item