Widowati, Wahyu and Kusuma, Hanna Sari W. and Arumwardana, Seila and Afifah, Ervi and Wahyuni, Cintani Dewi and Wijayanti, Cahyaning Riski and Maulana, Muhamad Aldi and Rizal, Rizal (2021) Corilagin Potential in Inhibiting Oxidative and Inflammatory Stress in LPS-induced Murine Macrophage Cell Lines (RAW 264.7). Iranian Journal of Basic Medical Sciences, 24. pp. 1656-1665. ISSN 2008-3874
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Abstract
Objective(s): Inflammation is thought to be the common pathophysiological basis for several disorders. Corilagin is one of the major active compounds which showed broad-spectrum biological and therapeutic activities, such as antitumor, hepatoprotective, anti-oxidant, and anti-inflammatory. This study aimed to evaluate the anti-oxidant and anti-inflammatory activities of corilagin in LPS�induced RAW264.7 cells. Materials and Methods: Anti-oxidant activities were examined by free radical scavenging of H2 O2 , NO, and *OH. The safe concentrations of corilagin on RAW264.7 were determined by MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] assay on RAW264.7 cell lines. The inflammation cells model was induced with LPS. The anti-inflammatory activities measured IL-6, TNF-α, NO, IL-1β, PGE-2, iNOS, and COX-2 levels using ELISA assay. Results: The results showed that corilagin had a significant inhibition activity dose-dependently in scavenging activities toward H2 O2 , *OH, and NO with IC50 values 76.85 µg/ml, 26.68 µg/ml, and 66.64 µg/ml, respectively. The anti-inflammatory activity of corilagin also showed a significant decrease toward IL-6, TNF-α, NO, IL-1β, PGE-2, iNOS, and COX-2 levels at the highest concentration (75 µM) compared with others concentration (50 and 25 µM) with the highest inhibition activities being 48.09%, 42.37%, 65.69%, 26.47%, 46.88%, 56.22%, 59.99%, respectively (P<0.05). Conclusion: Corilagin has potential as anti-oxidant and anti-inflammatory in LPS-induced RAW 264.7 cell lines by its ability to scavenge free radical NO, *OH, and H2 O2 and also suppress the production of proinflammatory mediators including COX-2, IL-6, IL-1β, and TNF-α in RAW 264.7 murine macrophage cell lines.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Anti-inflammatory, Anti-oxidant, Corilagin, LPS, RAW 264.7 |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 22 Nov 2022 02:11 |
| Last Modified: | 22 Nov 2022 02:11 |
| URI: | http://repository.maranatha.edu/id/eprint/31137 |
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