Widowati, Wahyu and Jasaputra, Diana Krisanti and Gunawan, Kamila Yashfa and Kusuma, Hanna Sari Widya and Arumwardana, Seila and Wahyuni, Cintani Dewi and Lister, I Nyoman Ehrich and Ginting, Chrismis Novalinda and Girsang, Ermi and Rizal, Rizal (2021) Turmeric Extract Potential Inhibit Inflammatory Marker in LPS-Stimulated Marcophage Cells. International Journal of Applied Pharmaceutics, 13 (3). ISSN 0975-7058
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Abstract
Objective: Inflammation can be induced by microbiological, chemical, physical factors and plays roles in inflammatory diseases. Turmeric (Curcuma longa L.) has been widely used to provide a diverse array of biological activities, including anti-inflammatory, antimicrobial, also antioxidant. The Turmeric extract (TE) anti-inflammatory potential was conducted using a Lipopolysaccharide (LPS)-induced RAW264.7 macrophage cell line by inhibiting inflammatory mediators especially IL-6, PGE-2, IL-1β, COX-2, TNF-α, iNOS, also NO level. Methods: The TE safe concentration in LPS-induced macrophage cell line was measured using MTS assay for further assay. The inflammatory markers (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO) were measured using ELISA assay and NO by the nitrate/nitrite colorimetric assay in LPSinduced RAW264.7 cell line. LPS induced inflammatory marker by increasing inflammatory marker (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO). Results: TE with 100 to 25 μg/ml, caused a significant reduction of cells viability, reaching only 30.27 % live cells. TE with lower concentrations (7.5; 5; 2.5 μg/ml) had no cytotoxic effect on macrophage cells (viability 117.31-131.08 %). LPS induction caused an increase in inflammatory cytokines IL-1β, PGE-2, IL-6, COX-2, TNF-α as well as iNOS and NO. Turmeric extract caused the reduction of the inflammatory cytokines in a dosedependent manner. Conclusion: The research resulted that TE has anti-inflammatory activity by decreasing IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, and NO level on LPS-induced RAW264.7 cells.
| Item Type: | Article |
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| Uncontrolled Keywords: | Inflammation, Turmeric, TNFα, IL-6, IL-1β |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 24 Mar 2022 05:22 |
| Last Modified: | 24 Mar 2022 05:22 |
| URI: | http://repository.maranatha.edu/id/eprint/30177 |
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