Hypoxic and Normoxic-Human Wharton’s Jelly Mesenchymal Stem Cell-Free Lysate for Anticancer Therapies

Widowati, Wahyu and Jasaputra, Diana Krisanti and Kusuma, Hanna Sari W. and Rizal, Rizal and Laksmitawati, Dian R. and Artie, Dwi Surya and Sholihah, Ika Adhani and Yusepany, Dewani Tediana and Lestari, Riyani and Handayani, Rr. Anisa Siwianti and Arumwardana, Seila and Murti, Harry and Bachtiar, Indra and Subangkit, Mawar (2021) Hypoxic and Normoxic-Human Wharton’s Jelly Mesenchymal Stem Cell-Free Lysate for Anticancer Therapies. Walailak Journal, 18 (9).

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Abstract

Cancer can be defined as a disease in which a group of uncontrolled cells growth by ignoring the rules of normal cell division. Mesenchymal Stem Cells (MSCs) are one of the promising cell-based therapies for anticancer therapies. Preceding research had disclosed that conditioned medium from hypoxic-treated human Wharton’s jelly mesenchymal stem cells (hWJMSCs-hypoCM) and normoxic-treated hWJMSCs (hWJMSCs-norCM) can inhibit the proliferation of various cancer cell lines and have no cytotoxic effect on normal cells. The present study, hence, measured the effect of a human Wharton’s jelly stem cells cell-free lysate (hWJMSCs-CL), cultured with hypoxic condition (hWJMSCs-hypoCL), and normoxic condition (hWJMSCs-norCL) towards several cancer cell lines including ovarian (SKOV3), cervical (HeLa), tongue squamosa (HSC3), and liver (HepG2) proliferation. Human mesenchymal stem cells (hMSCs), human fibroblast cells, and mouse fibroblast cells (NIH3T3) were used as control. Both hWJMSCs-CL grown in hypoxic and normoxic conditions could inhibit the proliferation of various cancer cell lines with median inhibitory concentration (IC50) 21.09 - 95.92 μg/mL and could cause low inhibition of the normal cells with IC50 409.19 - 799.74 μg/mL. The hWJMSCs-hypoCL and hWJMSC-norCL could inhibit various cancer and were not harmful to normal cells.

Item Type: Article
Uncontrolled Keywords: Anticancer, Cell-free lysate, Hypoxia, Mesenchymal stem cells, Wharton’s jelly
Subjects: R Medicine > R Medicine (General)
Depositing User: Perpustakaan Maranatha
Date Deposited: 01 Nov 2021 01:52
Last Modified: 01 Nov 2021 01:52
URI: http://repository.maranatha.edu/id/eprint/28004

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