Gong, Yue and Li, Hong Xu and Guo, Rui Hong and Widowati, Wahyu and Kim, Young Ho and Yang, Seo Young and Kim, Young Ran (2021) Anti-allergic Inflammatory Components from the Leaves of Piper crocatum Ruiz & Pav. Biological and Pharmaceutical Bulletin, 44 (2). pp. 245-250. ISSN 1347-5215
|
Text
59.Int Publikasi 2021-2 (Gong BPB-Wahyu FKUKM) Reviewer.pdf Download (5Mb) | Preview |
|
|
Text
59. Turnitin_Anti-allergic Inflammatory Components from the Leaves of Piper crocatum Ruiz & Pav.pdf Download (3060Kb) | Preview |
Abstract
Piper crocatum R uiz & Pav. ( P. crocatum), a traditional medicinal plant, has been shown to possess various pharmacological activities, including anticancer activity, antioxidant activity, antibacterial activity, anti-hyperglycemic activity, anti-allergic inflammatory activity and others. To identify the potential anti-allergic inflammatory effective constituents of P. crocatum, 13 single compounds were isolated from the methanol extract of P. crocatum leaves, and their structures were identified by contrasting their NMR spectroscopic data and previously published papers. First, the anti-allergic inflammatory activities of these single compounds were examined by accessing immune function related biomarkers such as nitric oxide (NO) and β-hexosaminidase. We found that the methanol extract and catechaldehyde (compound 1) potently suppressed NO production. Additionally, Western blot analysis showed that P. crocatum methanol extract and compound 1 suppressed the production of NO by reducing inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-induced RAW264.7 macrophages. Consistent with these observations, P. crocatum methanol extract and compound 1 remarkably decreased β-hexosaminidase release from RBL-2H3 cells stimulated with 2,4-dinitrophenylated bovine serum albumin (DNP-BSA)-specific immunoglobulin E (IgE) antibodies. Furthermore, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay indicated that P. crocatum methanol extract and compound 1 exhibited no cytotoxicity to RAW264.7 and RBL-2H3 cells. Based on these findings, compound 1 is suggested as an active anti-allergic inflammatory component of P. crocatum.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | allergic inflammation; Piper crocatum Ruiz & Pav.; catechaldehyde; nitric oxide; inducible nitric oxide synthase; β-hexosaminidase |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 15 Sep 2021 02:13 |
| Last Modified: | 14 Nov 2022 05:03 |
| URI: | http://repository.maranatha.edu/id/eprint/27818 |
Actions (login required)
 |
View Item |
