Cosmeceutical potentials of Curcuma mangga Val. extract in human BJ fibroblasts against MMP1, MMP3, and MMP13

Pujimulyani, Dwiyati and Suryani, Ch. Lilis and Setyowati, Astuti and Handayani, Rr. Anisa Siwianti and Arumwardana, Seila and Widowati, Wahyu and Maruf, Ali (2020) Cosmeceutical potentials of Curcuma mangga Val. extract in human BJ fibroblasts against MMP1, MMP3, and MMP13. Heliyon, 6.

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49. Cosmeceutical potentials of Curcuma mangga Val. extract in human BJ fibroblasts against MMP1, MMP3, and MMP13.pdf

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Abstract

Oxidativestress,thedisruptedoxidation-reductionmechanisminourbody,iscausedbytheexcessiveexposureof free radicalsandtheimpairedantioxidantdefensesthatcanaccelerateskinaging.Antioxidantscanbeobtained from nature,whichareavailablewidelyintherapeutic-richplants,suchaswhitesaffron(Curcuma mangga Val., denotedas C. mangga). Althoughmanypiecesofevidencerevealthat C. mangga contains anabundanceof phenoliccompoundsandhasantioxidativeeffects,itscosmeceuticalpotentialsremainunclear.Thepresentstudy aimed todisclosetheunexploredantiagingpotentialsof C. mangga extract (CME)inoxidativestress-induced human BJ fibroblasts withafocusoncollagenprotectionagainstpro-inflammatory mediatorsMMP1,MMP3, and MMP13.Theoxidativestress-inducedcellsweretreatedwithCMEandcurcuminatdifferentdoses.The resultsshowedthattreatmentusingCME(25 μg/mL) couldmaintainthecollagencontentsupto18.45 � 0.68 μg/ mL inH2O2-treated fibroblasts (only~26.63%reductionincollagencontents),whilethe figure forthenegative control wasthelowest(12.79 μg/mL), showingasignificant reductionincollagencontentsby49.13%.In addition,thegeneexpressionofpro-inflammatory MMPsarosesignificantly inBJ fibroblasts afteroxidativestress inductionusing200 μM H2O2, inwhichtheexpressionforMMP1,MMP3,andMMP13increasedby7.10,38.96, and 2.69times,respectively.Interestingly,CMEtreatment(100 μg/mL) couldeffectivelyinhibitMMP1,MMP3, and MMP13geneexpressionby3.65,34.62,and2.02times,respectively.Inconclusion,CMEshowedfavorable antiagingactivitiesinH2O2-treated humanBJ fibroblasts asconfirmed bythelowlevelsofgeneexpressionof MPP1, MMP3,andMMP13aftertreatmentwithCME.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Perpustakaan Maranatha
Date Deposited: 14 Sep 2021 22:01
Last Modified: 05 Nov 2021 08:16
URI: http://repository.maranatha.edu/id/eprint/27813

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