Anti-Inflammation Assay of Black Soybean Extract and Its Compounds on Lipopolysaccharide-Induced RAW 264.7 Cell

Widowati, Wahyu and Prahastuti, Sijani and Ekayanti, N.L.W. and Munshy, U.Z. and Kusuma, Hanna Sari W. and Wibowo, Satrio Haryo Benowo and Amalia, Annisa and Widodo, Wahyu Setia and Rizal, Rizal (2019) Anti-Inflammation Assay of Black Soybean Extract and Its Compounds on Lipopolysaccharide-Induced RAW 264.7 Cell. In: The 1st International Seminar on Smart Molecule of Natural Resources, 11-12 July 2019, Malang.

[img]
Preview
Text
44.Int Publikasi 2019-10 (Wahyu JPCS.-Wahyu FKUKM) Reviewer.pdf

Download (7Mb) | Preview
[img]
Preview
Text
44. Turnitin_Anti Inflammation Assay of Black Soybean Extract.pdf

Download (4004Kb) | Preview
[img]
Preview
Text
44.Int Publikasi 2019-10 (Wahyu JPCS-Wahyu FKUKM) Corr Author.pdf

Download (4Mb) | Preview

Abstract

Abstract. Inflammation response is related with various diseases. One of the useful therapeutic method to suppress inflammatory mediator synthesis is by application of compounds isolated from herbal medicine as treatment for inflammatory diseases. The aim of this study was to analyse the anti-inflammatory activity of black soybean extract (BSE), daidzein, and genistein trough in vitro analysis of inflammatory mediators such as prostaglandin 2 (PGE2) and cytokines interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α). Safety of samples was determined by viability test using MTS (3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4- sulfophenyl)-2H-tetrazolium). Concentration tested for viability assay were 40, 200, 1000 μg/mL for BSE, daidzein, and genistein. Anti-inflammation activity of samples was determined by ELISA quantification of PGE-2, TNF-α, and IL-1β in conditioned medium (CM) of supplemented pro-inflammatory activated RAW 264.7 cell. Inflammation on cells were induced by Lipopolysaccharide (LPS). BSE 1000 ug/ml, daidzein 1000 ug/ml, and genistein 1000 ug/ml treatments shows <80% cell viability average compared to control cell, indicating the treatments have cytotoxicity effect on RAW 264.7 cells. Hence, concentration used for treatments are 40 and 200 μg/mL for each sample. Genistein with concentration of 40 μg/ml treatment result shows highest anti-inflammatory activity which indicated from PGE-2, TNF-α, and IL-1β concentration. This study suggests that BSE, daidzein, and genistein with concentration of 40 and 200 μg/ml were safe to use for RAW 264.7 cell and genistein with concentration of 40 μg/ml have the best anti-inflammatory activity compared to daidzein and BSE.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: inflammation, black soybean, RAW 264.7 Cell, PGE-2, TNF-α, IL-1β
Subjects: R Medicine > R Medicine (General)
Depositing User: Perpustakaan Maranatha
Date Deposited: 14 Sep 2021 15:29
Last Modified: 14 Nov 2022 02:54
URI: http://repository.maranatha.edu/id/eprint/27805

Actions (login required)

View Item View Item