Dental Calculus as a Potential Biosource for Human Papillomavirus Detection in Oral Squamous Cell Carcinoma

Pranata, Natallia and Maskoen, Ani Melani and Sahiratmadja, Edhyana and Widyaputra, Sunardhi (2020) Dental Calculus as a Potential Biosource for Human Papillomavirus Detection in Oral Squamous Cell Carcinoma. Asian Pacific Journal of Cancer Prevention, 21 (10). pp. 3093-3097.

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Abstract

Objective: The infection of human papillomaviruses (HPVs) plays a role in the development of oral squamous cell carcinoma (OSCC). A poor oral hygiene and dental calculus may cause the infection to persist. Therefore, this study aimed to assess whether this dental calculus could serve as a potential biosource in early detection of HPVs in patients with OSCC. Methods: DNA was isolated from the dental calculus of people diagnosed with OSCC, and MY09/11 primer set was used to detect the presence of HPV. The positive samples were further sequenced and aligned using megablast NCBI BLAST tool to identify the HPV genotype. Results: Electrophoresis examination showed that 4 of 14 samples collected (29%) had a clear single band, of which three had 97% to 99% similarity to a high-risk genotype HPV-58. Meanwhile, the other sample had 99% similarity to an unclassified papillomaviridae. Conclusion: Dental calculus is a promising source of HPV in oral cavity and could be used as a biomarker for early detection.

Item Type: Article
Uncontrolled Keywords: Dental informatics- bioinformatics- microbial genetics- oral carcinogenesis- oral pathology
Subjects: R Medicine > RK Dentistry
Depositing User: Perpustakaan Maranatha
Date Deposited: 27 Apr 2021 06:54
Last Modified: 27 Apr 2021 06:54
URI: http://repository.maranatha.edu/id/eprint/27620

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