Pranata, Natallia and Maskoen, Ani Melani and Sahiratmadja, Edhyana and Widyaputra, Sunardhi (2020) Dental Calculus as a Potential Biosource for Human Papillomavirus Detection in Oral Squamous Cell Carcinoma. Asian Pacific Journal of Cancer Prevention, 21 (10). pp. 3093-3097.
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Abstract
Objective: The infection of human papillomaviruses (HPVs) plays a role in the development of oral squamous cell carcinoma (OSCC). A poor oral hygiene and dental calculus may cause the infection to persist. Therefore, this study aimed to assess whether this dental calculus could serve as a potential biosource in early detection of HPVs in patients with OSCC. Methods: DNA was isolated from the dental calculus of people diagnosed with OSCC, and MY09/11 primer set was used to detect the presence of HPV. The positive samples were further sequenced and aligned using megablast NCBI BLAST tool to identify the HPV genotype. Results: Electrophoresis examination showed that 4 of 14 samples collected (29%) had a clear single band, of which three had 97% to 99% similarity to a high-risk genotype HPV-58. Meanwhile, the other sample had 99% similarity to an unclassified papillomaviridae. Conclusion: Dental calculus is a promising source of HPV in oral cavity and could be used as a biomarker for early detection.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Dental informatics- bioinformatics- microbial genetics- oral carcinogenesis- oral pathology |
| Subjects: | R Medicine > RK Dentistry |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 27 Apr 2021 06:54 |
| Last Modified: | 18 Apr 2022 10:02 |
| URI: | http://repository.maranatha.edu/id/eprint/27620 |
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