Sandhlutami, Ni Made Dwi and Moordiani, Moordiani and Laksmitawati, Dian R. and Fauziah, Nurul and Maesaroh, Maesaroh and Widowati, Wahyu (2017) In Vitro Assesment of Anti-Inflammatory Activities of Coumarin and Indonesian Cassia Extract in RAW264.7 Murine Macrophage Cell Line. Iranian Journal of Basic Medical Sciences, 20 (1). pp. 99-106. ISSN 2008-3874
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Abstract
Objective(s): Inflammation is an immune response toward injuries. Although inflammation is healing response, but in some condition it will lead to chronic disease such as rheumatoid arthritis, inflammatory bowel disease, atherosclerosis, Alzheimer’s and various cancer. Indonesian cassia (Cinnamomum burmannil C. Nees & T. Ness) known to contain coumarin, is widely used for alternative medicine especially as an anti-inflammatory. This study was conducted to determine the antiinflammatory properties of coumarin and Indonesian cassia extract (ICE) in LPS-induced RAW264.7 cell line. Materials and Methods: The cytotoxic assay of coumarin and ICE against RAW264.7 cells was conducted using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium). The anti-inflammatory potential was determined using LPS-induced RAW 267.4 macrophages cells to measure inhibitory activity of compound and ISEon production of nitric oxide (NO), prostaglandin E2 (PGE2), and also cytokines such as interleukin-6 (IL-6), interleukin-1β (IL-1β) and TNF-α. Results: Coumarin 10 μM and ICE 10 μg/ml were nontoxic to the RAW264.7 cells. Both of coumarin and ICE were capable to reduce the PGE2, TNF-α, NO, IL-6, and IL-β level in LPS-induced RAW264.7 cells. Coumarin had higher activity to decrease PGE2 and TNF-α, whilst ICE had higher activity to inhibit NO, IL-6, and IL-β levels. Conclusion: Coumarin and ICE possess anti-inflammatory properties through inhibition of PGE2 and NO along with pro-inflammatory cytokines TNF-α, IL-6, IL-1β production.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Coumarin Cytokines Indonesian cassia extract Inflammation RAW264.7 |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 06 Oct 2020 09:21 |
| Last Modified: | 14 Nov 2022 03:59 |
| URI: | http://repository.maranatha.edu/id/eprint/27390 |
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