Widowati, Wahyu and Jasaputra, Diana Krisanti and Onggowidjaja, Philips and Sumitro, Sutiman B. and Widodo, Mochammad Aris and Afifah, Ervi and Rihibiha, Dwi Davidson and Rizal, Rizal and Amalia, Annisa and Kusuma, Hanna Sari W. and Murti, Harry and Bachtiar, Indra (2019) Effects of Conditioned Medium of Co-Culture IL-2 Induced NK Cells and Human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) on Apoptotic Gene Expression in a Breast Cancer Cell Line (MCF-7). Journal of Mathematical and Fundamental Sciences, 51 (3). pp. 205-224. ISSN 2337-5760
|
Text
41.Int Publikasi 2019-7 (Wahyu JMFS-Wahyu FKUKM) Reviewer.pdf Download (6Mb) | Preview |
|
|
Text
41.Int Publikasi 2019-7 (Wahyu JMFS-Wahyu FKUKM) Corr Author.pdf Download (737Kb) | Preview |
|
|
Text
41.Turnitin_Int Publikasi 2019-7 (Wahyu JMFS-Wahyu FKUKM).pdf Download (4017Kb) | Preview |
Abstract
Abstract. Breast cancer (BC) is the most prevalent type of cancer among women and one of the major causes of cancer mortality in women. Metastasis in breast cancer (BC) occurs due to immunosurveillance deficiency, including impairment of natural killer (NK) cell maturation. Conditioned medium (CM) from human Wharton’s jelly mesenchymal stem cells (hWJMSC-CM) is known to possess anticancer activity. The CM of co-culture of human recombinant IL-2 treated NK cells and hWJMSCs is expected to boost anticancer activity toward BC cells which can be analyzed from the effect of CM towards secretion of effector molecules and expression of BC cell apoptosis-related genes, and cytotoxic granules in human recombinant IL-2 treated NK (IL-2 NK) and hWJMSCs (IL-2 hWJMSCs). TNF-α, IFN-γ, perforin, granzyme were measured by ELISA, while the inhibition of cell proliferation was measured by MTS assay and BC cell apoptosis by flow cytometry and apoptotic gene expression by RTPCR. CM from co-cultured hWJMSCs and IL-2 NK cells inhibited NK and BC cell proliferation, increased expression of Bax and p53 and decreased the number of Bcl-2 in BC cells. In conclusion, CM of co-culture IL-2 treated NK cells and hWJMSCs induce apoptosis in BC cells as indicated by increased Bax and p53 expression and decreased Bcl-2 expression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | breast cancer; conditioned medium; human Wharton’s jelly mesenchymal stem cells; interleukin-2; natural killer cells. |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 06 Oct 2020 06:13 |
| Last Modified: | 15 Nov 2022 05:27 |
| URI: | http://repository.maranatha.edu/id/eprint/27384 |
Actions (login required)
 |
View Item |
