Gunadi, Julia Windi and Tarawan, Vita Murniati and Setiawan, Iwan and Goenawan, Hanna and Ratnawati, Hana and Limyati, Yenni and Adhika, Oeij Anindita and Santoso, Andreas Warsono and Lesmana, Ronny and Supratman, Unang (2019) Adaptation of Aerobic Training Essentially Involved Autophagy, Mitochondrial Marker and Muscle Fibre Genetic odulation in Rat Cardiac Muscles. Journal of Animal Physiology and Animal Nutrition. pp. 1-10.
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Abstract
Information about the role of moderate acute treadmill training in modulating autophagy and mitochondrial markers that might be correlated with alteration of muscle fibre gene expression in rat cardiac muscles is very limited. In this present study, the researchers divided twenty male Wistar rats into four groups: sedentary control, 3, 6 and 15 days and subjected them to treadmill training with moderate intensity (20 m/ min), 30 min each day. RNA was extracted from cardiac muscles and stored in temperature of −80°C. Specific primers were utilized for semi‐quantitative PCR. Treadmill training decreased autophagy‐related gene expression (LC3, p62) and upper stream signalling of autophagy (PIK3CA, Akt and mTOR) in 3 and 6 d, but stimulated gene expression of mitochondrial markers (PGC1α, Cox1, Cox2 and Cox4) in 15 days. αMHC gene expression increased while βMHC gene expression decreased in 15 days. In line with this, autophagy‐related genes increased in 3 and 6 days and returned to baseline in 15 days. The increment in mitochondrial gene expression might be correlated with shifting gene expression of αMHC and βMHC in 15 days. Taken together, acute adaptation in cardiac muscles is stimulated by genetic modulation of autophagy, mitochondrial marker and muscle fibre that may explain physiological cardiac adaptation after training. This study can be used as a reference for optimizing performance in period of cardiac muscle adaptation stimulated by treadmill training.
| Item Type: | Article |
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| Uncontrolled Keywords: | adaptation, autophagy, cardiac, mitochondria, myosin heavy chain, training |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 08 Sep 2020 02:17 |
| Last Modified: | 08 Sep 2020 02:18 |
| URI: | http://repository.maranatha.edu/id/eprint/27350 |
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