Cytotoxic Activity of Detam 1 Soybean and Jati Belanda (Guazuma ulmifolia) Extracts on SV40 MES 13 Cell

Hidayat, Meilinah and Prahatusti, Sijani and Soemardji, Andreanus A. and Rihibiha, Dwi Davidson and Afni, Merry and Afifah, Ervi and Widowati, Wahyu (2016) Cytotoxic Activity of Detam 1 Soybean and Jati Belanda (Guazuma ulmifolia) Extracts on SV40 MES 13 Cell. In: The International Seminar on Pharmacology and Clinical Pharmacy 2016, 1-2 September 2016, Bandung.

[img]
Preview
Text
Cytotoxic Activity of Detam 1 Soybean and Jati Belanda (Guazuma ulmifolia) Extracts on SV40 MES 13 Cell.pdf

Download (8Mb) | Preview

Abstract

The amount of Diabetes mellitus patients in Indonesia is predicted to be continually increasing. Lifestyle and pancreatic dysfunction in producing insulin, have been known as the main causes. Furthermore, diabetes mellitus type 1 and 2 can progressively develop, resulting in complication known as “diabetic glomerulosclerosis”. Diminished glomerular basement membrane causes failure in urine filltration, that causes presence of albumin in urine. Natural sources in Indonesia has been reported to contain bioactive compounds that possess medicinal properties. Jati Belanda (Guazuma ulmifolia) and Detam 1 soybean has been known to act as antiobesity and antidiabetes due to presence of its secondary metabolite. Objectives: This study aimed to determine cytotoxic activity of extract of Detam 1 soybean, Jati Belanda, and combination of extracts, toward SV40 MES 13 cell (Glomerular Mesangial Kidney, Mus musculus). Methods: Cytotoxic activity was measured with 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium(MTS) assay. Concentrations used were 3.125, 6.25, 12.5, 25, 50, and 100 µg/mL. Results: Both Jati Belanda and Detam 1 soybean extract at concentration of 3.125, 6.25, and 12.5 µg/mL showed no toxicity toward SV40 MES 13 cell, as indicated by >90% viable cells, whilst at concentration of 25, 50, and 100 µg/mL showed toxicity, as indicated by <90% viable cells. The combination of extracts showed no toxicity only at low concentration (3.125 µg/mL). Conclusion: Thus, Jati Belanda and Detam 1 soybean extract at concentration of 3.125, 6.25, 12.5 µg/mL and the combination of extracts at 3.125 µg/mL were considered safe toward SV40 MES 13 cell.

Item Type: Conference or Workshop Item (Paper)
Uncontrolled Keywords: Diabetic glomerulosclerosis, Jati Belanda, Detam 1 Soybean, SV40 MES 13 cell.
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine > 10 School of Medicine
Depositing User: Perpustakaan Maranatha
Date Deposited: 14 Nov 2019 09:10
Last Modified: 14 Nov 2019 09:10
URI: http://repository.maranatha.edu/id/eprint/27091

Actions (login required)

View Item View Item