Induction of Matrix Metalloproteinases in Chondrocytes by Interleukin IL-1β as an Osteoarthritis Model

Afifah, Ervi and Mozef, Tjandrawati and Sandra, Ferry and Arumwardana, Seila and Rihibiha, Dwi Davidson and Nuvus, Hayatun and Rizal, Rizal and Amalia, Annisa and Bachtiar, Indra and Murti, Harry and Widowati, Wahyu (2018) Induction of Matrix Metalloproteinases in Chondrocytes by Interleukin IL-1β as an Osteoarthritis Model. Journal of Mathematical and Fundamental Sciences, 51 (2). pp. 103-111. ISSN 2337-5760

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Abstract

Osteoarthritis (OA) is a chronic disease of the joints and bones due to trauma or other joint-related diseases (secondary). Synovial inflammation commonly causes disturbance in joint homeostasis, which is associated with OA. Enzymes such as aggrecanase and metalloproteinase generate cartilage damage, mediated by tumor necrosis factor (TNF-α) and interleukin (IL)-1. Proinflammatory cytokines, including TNF-α, IL-1β, and IL-6, are responsible for regulation of the extracellular matrix, cartilage degradation, and apoptosis of chondrocytes. This study aimed to observe the cell viability and expression level of matrix metalloproteinases (MMP-1 and MMP-3) and tissue inhibitor metalloproteinases (TIMP-1 and TIMP-2) in human chondrocyte cells (CHON- 002) induced by IL-1β. CHON-002 was induced with IL-1β (0.1, 1 and 10 ng/mL) as an OA model. The viability of the cells was measured with a 3-(4,5- dimethylthiazol-2-yl)-5-(3-carboxyme-thoxyphenyl)-2-(4-sulfophenyl)-2H-tetra zolium (MTS) assay, while expression of MMP-1, MMP-3, TIMP-1, and TIMP- 2, was evaluated by RT-PCR. The viability of IL-1β-induced CHON-002 (CHON-002- IL-1β) cells at day 1 and 5 showed that treatment with up to 10 ng/mL of IL-1β was not toxic. Expression of TIMP-1 and TIMP-2 in CHON- 002-IL-1β was lower compared to control, while that of MMP-1 and MMP-3 was higher compared to control. These results indicate that CHON-002 treated with 10 ng/mL IL-1β has expression patterns consistent with chondrocyte damage, so the CHON-002-IL-1β system may serve as a model for MMP induction in OA.

Item Type: Article
Uncontrolled Keywords: interleukin; mesenchymal stem cell; metalloproteases; MMP genes; vosteoarthritis.
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine > 10 School of Medicine
Depositing User: Perpustakaan Maranatha
Date Deposited: 29 Oct 2019 01:11
Last Modified: 29 Oct 2019 01:11
URI: http://repository.maranatha.edu/id/eprint/27084

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