Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin

Geeta, Geeta and Widodo, Wahyu Setia and Widowati, Wahyu and Ginting, Chrismis Novalinda and I Nyoman, Ehrich Lister and Armansyah, Adek and Girsang, Ermi (2019) Comparison of Antioxidant and Anti-collagenase Activity of Genistein and Epicatechin. Pharmaceutical Sciences and Research, 6 (2). pp. 111-117. ISSN 2477-0612

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Abstract

Background: Skin aging is a complex natural process caused by both intrinsic or genetically programmed aging and extrinsic aging caused by environmental factors, such as free radical. The use of antioxidant and anti-collagenase to prevent the aging proses has been known. Natural compounds from plants are one of the sources of antioxidant and anti-collagenase that has ability to prevent aging. Genistein and Epicatechin are the major phenolic compound found in G. max. Objectives: This research was to evaluate the antiaging potential of genistein and epicatechin through antioxidant activity assay (ABTS-reducing activity assay) and collagenase inhibitory activity assay. Methods: Antioxidant analysis of Genistein and Epicatechin was performed by 2,2’- azinobis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) reducing activity Assay. Antiaging assay was conducted through inhibitory of collagenase, one of important enzyme in aging process. Results: ABTS- reducing activity assay showed that both compounds had great ABTS-reducing activity in which epicatechin had better activity than genistein. Epicatechin had low value of IC50 ABTS-reducing activity around 14.39 μg/ml better than genistein with IC50 about 43.17 μg/ml. In terms of collagenase inhibitory activity assay, epicatechin had lower value of IC50 (9.08+-3.46 ug/ml), better than genistein (98.74+-4.25 ug/ml). Conclusion: Epicatechin had higher antioxidant and anti- collagenase activity compared to Genistein.

Item Type: Article
Uncontrolled Keywords: ABTS-reducing activity; anti-collagenase; genistein; epicatechin
Subjects: R Medicine > R Medicine (General)
Divisions: Faculty of Medicine > 10 School of Medicine
Depositing User: Perpustakaan Maranatha
Date Deposited: 29 Oct 2019 00:49
Last Modified: 29 Oct 2019 00:49
URI: http://repository.maranatha.edu/id/eprint/27082

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