Effect of Steamed Broccoli Juice (Brassica oleraceae L. var. italica) to the Serum Interleukin B8 Level in Colitis Murine Model

Samuel, Lukas Mulyono and A.S., Francisca and Khiong, Khie (2015) Effect of Steamed Broccoli Juice (Brassica oleraceae L. var. italica) to the Serum Interleukin B8 Level in Colitis Murine Model. The Indonesian Journal of Gastroenterology Hepatology and Digestive Endoscopy, 16 (3). pp. 148-152. ISSN 1411-4801

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Abstract

Background: Ulcerative colitis (UC) is a bowel inflammation that happens in the colon and rectum. In UC there is an increased level of inflammatory mediators, one of which is interleukin-8 (IL-8), the main chemoattactant for polymorphonuclear (PMN) inflammatory cells causing inflammation to worsen. Broccoli (Brassica oleraceae L. var. italica) contains sulforaphane which can inhibit nuclear factor kappa B (NF-kB), resulting in the decrease of IL-8. This study was performed to identify the effect of steamed broccoli juice to the serum interleukin-8 level in murine model of colitis. Method: This study was a true experimental laboratory study using complete randomized design. Experimental animals being used were 28 murines with Balb/c strain and were divided into 4 intervention groups. Serum IL-8 level was measured by using enzyme-linked immunosorbent assay (ELISA) method. Data analysis was performed using analysis of variance (ANOVA) test, followed by multiple comparisons Tukey honest significant difference (HSD) test (a=0.05) and p<=0.05. Results: The highest mean of IL-8 level was found in the positive control group. This study also showed highly significant difference (p<0.001) of IL-8 level between positive control group and treatment group. Conclusion: Steamed broccoli juice could decrease serum interleukin-8 level in murine model of colitis.

Item Type: Article
Uncontrolled Keywords: ulcerative colitis, sulforaphane, interleukin-8
Subjects: R Medicine > R Medicine (General)
Depositing User: Perpustakaan Maranatha
Date Deposited: 23 Sep 2016 03:39
Last Modified: 23 Sep 2016 03:39
URI: http://repository.maranatha.edu/id/eprint/21038

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