Modulation of Adipogenesis-Related Gene Expression by Ethanol Extracts of Detam 1 Soybean and Jati Belanda Leaf in 3T3-L1 Cells

Hidayat, Meilinah and Prahastuti, Sijani and Fauziah, Nurul and Maesaroh, Maesaroh and Balqis, Balqis and Widowati, Wahyu (2016) Modulation of Adipogenesis-Related Gene Expression by Ethanol Extracts of Detam 1 Soybean and Jati Belanda Leaf in 3T3-L1 Cells. Bangladesh Journal of Pharmacology (11). pp. 697-702. ISSN 1991-0088

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Abstract

In this study, we evaluated the effects of ethanol extracts of Detam 1 soybean, Jati belanda leaf, and the combination toward expression of peroxisome proliferator-activated receptor gamma (PPAR), CCAAT/enhancer-binding protein alpha (C/EBP), and stearoyl-CoA desaturase 1 (SCD1) genes in 3T3- L1 cells as anti-adipogenesis and anti-obesity. The differentiation of 3T3-L1 cells into adipocyte was conducted using induction medium consist of Dulbecco's Modified Eagle's Medium, 3-isobutyl-1-methylxanthine, insulin, dexamethasone, and fetal bovine serum. The expression of PPAR, C/EBP, and SCD1 gene was measured using real-time quantitative polymerase chain reaction (qPCR). Ethanol extract of Jati belanda at a concentration of 50 μg/ mL was most effective to reduce PPAR, C/EBP, and SCD1 gene expression in 3T3-L1 cells. Ethanol extract of Detam 1 soybean failed to reduce PPAR gene expression, whilst in the concentration of 50 μg/mL it was able to significantly reduce the C/EBP and SCD1 gene expression. Both ethanol extracts of Detam 1 soybean and Jati belanda have potential as antiadipogenesis and anti-obesity by suppressing adipogenesis-related gene expression, particularly C/EBP and SCD1.

Item Type: Article
Subjects: R Medicine > R Medicine (General)
Depositing User: Perpustakaan Maranatha
Date Deposited: 03 Aug 2016 08:18
Last Modified: 03 Aug 2016 08:18
URI: http://repository.maranatha.edu/id/eprint/20716

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