Selective Cytotoxic Potential of IFN-y and TNF-a on Breast Cancer Cell Lines (T47D and MCF7)

Widowati, Wahyu and Murti, Harry and Jasaputra, Diana Krisanti and Sumitro, Sutiman B. and Widodo, M. Aris and Fauziah, Nurul and Maesaroh, Maesaroh and Bachtiar, Indra (2016) Selective Cytotoxic Potential of IFN-y and TNF-a on Breast Cancer Cell Lines (T47D and MCF7). Asian Journal of Cell Biology (11). pp. 1-12. ISSN 1814-0068

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Background: Breast cancer is one of the most common life threatening diseases worldwide and it is the leading cause of death from cancer in women. The effectiveness of current cancer therapies is low, even though it requires expensive cost. Therefore, the development of the more efficient therapy is highly needed. Objective: This research was conducted to evaluate the effect Tumour Necrosis Factor alpha (TNF-") and Interferon gamma (IFN-() as anticancer agents against breast cancer cells (T47D, MCF7) and selectivity of cytotoxic effect towards human Wharton’s Jelly Mesenchymal Stem Cells (hWJMSCs) to produce hWJMSCs-Conditioned Medium (CM), hWJMSCs-Cell Lysate (CL) and hWJMSCs which potential used as anticancer candidates after induced by TNF" or IFN(. Methodology: The hWJMSCs were isolated from umbilical cord and characterized by its surface marker phenotype and its multipotent differentiation potential. The breast cancer cell lines (T47D and MCF7) were treated by TNF-" and IFN-( and its cytotoxic activity was observed by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium (MTS) viability assay. The cytotoxic effects of TNF-" and IFN-( toward hWJMSCs were also observed using the same method. Results: Flow cytometry analysis showed that hWJMSCs for early passage (P4) were positive for hMSCs marker CD105, CD73 and negative for CD34, CD45, CD14, CD19 and HLA-II. It also showed the osteogenic, adipogenic and chondrogenic differentiation. The effect of TNF-" and IFN-( against MCF7 cells exhibited that the cytokines decreased the cell viability in a dose dependent manner. The IC50 value of TNF-" and IFN-( against breast cancer cell lines were found higher for T47D than MCF7. Conclusion: The TNF-" and IFN-( inhibit the cell growth of breast cancer cells due to apoptotic or necrotic cells but it non-toxic against hWJMSCs.

Item Type: Article
Uncontrolled Keywords: Breast cancer, TNF-α, IFN-γ, hMSCs, cytotoxic, umbilical cord
Subjects: R Medicine > R Medicine (General)
Depositing User: Perpustakaan Maranatha
Date Deposited: 03 Aug 2016 07:59
Last Modified: 03 Aug 2016 07:59

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