Widowati, Wahyu and Widyanto, Rachma Micho and Laksmitawati, Dian R. and Erawijantari, Pande Putu and Wijaya, Laura and Sandra, Ferry (2015) Phytochemical, Free Radical Scavenging and Cytotoxic Assay of Cucumis Melo L. Extract and β-Carotene. Journal of Advanced Agricultural Technologies, 2 (2). pp. 114-119. ISSN 2373-423X
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Abstract
Abstract—Deaths from cancer worldwide are estimated to continue rising. Free radicals are toxic to cellular components. It is known that they cause DNA damage, contribute to DNA instability and mutation, thus favor carcinogenesis. This research was conducted to determine the activity of Cucumis melo extract and β-carotene in antioxidative and cytotoxic potencies. The research was done by examining the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity assay. The cytotoxic potency was determined by the MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay on HeLa, HepG2 and NIH3T3 cell lines. β-carotene exhibited more active DPPH free radical scavenging activity compared with C. melo extract. C. melo extract showed more active anti-cancer both in HeLa (IC50: 23.649 μg/mL) and HepG2 (IC50: 110.403 μg/mL) cancer cells. C. melo extract (IC50: 16,670.404 μg/mL) and β-carotene (IC50: 50,645.994 μg/mL) had low cytotoxicity in NIH3T3 fibroblast. C. melo extract has lower antioxidant activity, but higher cytotoxic potency compared with β-carotene.
| Item Type: | Article |
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| Uncontrolled Keywords: | antioxidant, free radical, melon, cucumis melo, β-carotene, cancer, cytotoxic. |
| Subjects: | R Medicine > R Medicine (General) |
| Depositing User: | Perpustakaan Maranatha |
| Date Deposited: | 22 Sep 2015 10:00 |
| Last Modified: | 22 Sep 2015 10:00 |
| URI: | http://repository.maranatha.edu/id/eprint/15433 |
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